Coding

Part:BBa_K1742004:Design

Designed by: Ivn Casas Rodrigo   Group: iGEM15_Valencia_UPV   (2015-09-04)


PhiC31 Plant codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 421
    Illegal NheI site found at 591
    Illegal NheI site found at 1633
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 79
    Illegal BamHI site found at 816
    Illegal BamHI site found at 846
    Illegal BamHI site found at 932
    Illegal BamHI site found at 942
    Illegal XhoI site found at 773
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1303
    Illegal SapI.rc site found at 1628


Design Notes

It has a nuclear location sequence (NLS) and its codons are optimized for plants.


Source

Synthetic

References